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General description
Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units
Preparation Note
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.
Other Notes
Kornberg, A. 1995. Methods Enzymol.1, 441
One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4
One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| description | Merck USA index - 14, 5334 |
| Quality Level | 100 |
| form | lyophilized |
| specific activity | ≥300 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,desiccated |
| impurities | ≤0.01% PK, MK,≤0.03% GPT, GOT,≤0.1% MDH |
| pI | 4.6 |
| solubility | aqueous buffer: soluble |
| shipped in | ambient |
| storage temp. | −20°C |
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¥ 2622.09
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¥ 1259.40
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¥ 2013.69
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¥ 1509.00
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