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General description
Trinucleotide repeat-containing gene 6A, 6B, and 6C proteins (UniProt: Q8NDV7; Q9UPQ9; Q9HCJ0) are encoded by the TNRC6A, TNRC6B, and TNRC6C genes (Gene ID: 27327, 23112, and 57690), respectively in human. TNRC6 proteins are ubiquitously distributed and play a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). TNRC6 proteins are required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. They also serve as a scaffolding proteins and associate with argonaute proteins bound to partially complementary mRNAs. They can simultaneously recruit CCR4-NOT and PAN deadenylase complexes. TNRC6 proteins are predominantly found in the cytoplasm under steady state conditions, however, they are also found in the nucleus and their nuclear import is mediated via the importin pathways. TNRC6A is shown to posses both nuclear localization (NLS) as well a nuclear export signal (NES). In case of mutated NES, TNRC6A is shown to be retained in the nucleus. TNRC6 proteins contain RRM domains (aa 1781-1853; 1648-1720; and 1563-1632 for TNRC6A, 6B, and 6C, respectively). Clone 7A9 recognizes all three TNRC6 proteins. (Ref.: Schraivogel, D., et al. (2015). Nucleic Acids Res. 43(15); 7447-7461).
~175-210 kDa observed; 210.30,194.00, 175.96 kDa for TNRC6A, 6B, and 6C, respectively. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Recombinant RRM domain regions from human TNRC6A, TNRC6B, and TNRC6C.
Application
Anti-TNRC6ABC, clone 7A9, Cat. No. MABE1919, is a highly specific rat monoclonal antibody that targets TNRC6 and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Immunofluorescence Analysis: A representative lot detected TNRC6ABC in Immunofluorescence applications (Schraivogel, D., et. al. (2015). Nucleic Acids Res. 43(15):7447-61).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TNRC6ABC in Immunoprecipitation applications (Quevillon Huberdeau, M., et. al. (2017). EMBO J. 36(14):2088-2106).
Research Category
Epigenetics & Nuclear Function
Biochem/physiol Actions
Clone 7A9 is a rat monoclonal antibody that detects human Trinucleotide repeat-containing gene 6A, 6B, and 6C proteins. It target epitopes with in RRM domains.
Physical form
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Induced Stably transfected HEK293 cell lysates.
Western Blotting Analysis: 4 µg/mL of this antibody detected TNRC6 in lysate from stably induced HEK 293 cells transfected with TNRC6A, but not in uninduced HEK 293 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7A9, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunofluorescence: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001020014,NP_001136112,NP_001317449 |
| UniProt accession no. | Q8NDV7,Q9HCJ0,Q9UPQ9 |
| target post-translational modification | unmodified |
| Gene Information | human ... TNRC6B(23112) |
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