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General description
Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-RAINBOW antibody is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. Anti-RAINBOW antibody detects all marker proteins in commercially available RAINBOW markers tested and lacks cross-reactivity with unstained proteins in whole cell lysates of a variety of species. It displays equal detection of Remazol-dye stained proteins: phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).
Immunogen
Purified Remazol Brilliant Blue R (RBBR)-BSA, followed by RO16-Chymotrypsin, RGY-Catalase and RF3B-Lysozyme.
Application
Anti-RAINBOW, HRP-conjugated, Cat. No. MABC1690H, is a mouse monoclonal antibody that recognizes four different Remazol dye-conjugated proteins and can be used to visualize prestained molecular weight markers in Western Blotting.
Research Category
Secondary & Control Antibodies
Western Blotting Analysis: A representative lot detected Remzaol-dye prestained protein standards in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).
Western Blotting Analysis: A 1:500 and 1:1,000 dilution from a representative lot detected protein standard.
Biochem/physiol Actions
Anti-RAINBOW, HRP-conjugated is a mouse monoclonal antibody that can be used as a universal tool for visualizing prestained protein molecular weight markers in Western blot analysis. It recognizes and cross-reacts with comparable affinity with four different Remazol dye immunogens.
Physical form
Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS with StabilZyme® HRP Conjugate Stabilizer and preservative.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Blue Pre-Stained Protein Standard.
Western Blotting Analysis: A 1:10,000; 1:20,000; 1:40,000; 1:80,000 dilution of this antibody detected Remzaol-dye prestained protein standards.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
StabilZyme is a registered trademark of SurModics, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | peroxidase conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | monoclonal |
| species reactivity (predicted by homology) | all |
| packaging | antibody small pack of 25 μg |
| technique(s) | western blot: suitable |
| isotype | IgG1κ |
| shipped in | ambient |
| target post-translational modification | unmodified |
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| 重量(kg) |
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