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- 规格参数
- 包装参数
General description
180 kDa
Immunogen
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 686 in human erbB2
Application
Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8 is an antibody against phospho-erbB2 (Thr686) for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
Kinases & Phosphatases
Biochem/physiol Actions
Recognizes phosphorylated erbB2 on Threonine 686
Physical form
Format: Purified
In 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol.
Subsequent thiophilic adsorption and size exclusion chromatography
Preparation Note
2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Routinely evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 7F8, monoclonal |
| species reactivity | mouse, human, canine |
| manufacturer/tradename | Upstate® |
| technique(s) | ELISA: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_001005862.1,NM_004448.2 |
| UniProt accession no. | P04626 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pThr686) |
| Gene Information | human ... ERBB2(2064) mouse ... Erbb2(13866) |
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