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- 规格参数
- 包装参数
General description
Digitonin is a non-ionic steroid saponin derived from Digitalis purpurea. Historically, digitonin has been used to solubilize receptors and permeabilize membranes for sample preparation and analysis. Digitonin interacts with cholesterol creating a digitonin-cholesterol complex, which leads to subsequent removal of cholesterol and cellular membrane permeabilization. Therefore, cellular compartments composed of low amounts of cholesterol are protected from digitonin effects, particularly the nuclear membrane.
Due to digitonin′s mechanism of action, many other epigenetic applications utilize digitonin for nuclear extraction. For example, assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-Seq) is a valuable method to map open chromatin sites and assess the epigenetic landscape. In combination with other detergents, a low concentration of digitonin is employed to isolate the nuclear pellet followed by digestion of the nuclei with an increased concentration of digitonin5. In our ChIC (Chromatin Immuno-Cleavage) Kits (part #s: CHR100-102), digitonin is utilized for antibody targeting of micrococcal nuclease for chromatin tagging.
Other Notes
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
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¥ 2622.09
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¥ 1451.54
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¥ 2068.25
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¥ 3275.78
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瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmPES滤膜，孔径0.45um,适合瓶颈尺寸33mm，12/箱，295-3345，Nalgene，Thermofisher，赛默飞世尔¥ 1938.54
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