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General description
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515 ) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
Biochem/physiol Actions
Primary Target
Nitric oxide (NO) fluorescent indicator
Physical form
A 5 mM solution in DMSO.
Preparation Note
Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
Other Notes
Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.
Legal Information
Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
| Quality Level | 100 |
| assay | ≥98% (HPLC) |
| form | solution |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,protect from light |
| color | pale yellow |
| fluorescence | λex ~500 nm,λem ~515 nm |
| storage temp. | −20°C |
| SMILES string | Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC |
| InChI | 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3 |
| InChI key | BEVHTVRRVVEMEF-UHFFFAOYSA-N |
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¥ 57595.03
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