Anti-14-3-3σ Antibody, clone CS112-2A8，200UG，05-632，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

30 kDa

Immunogen

GST fusion protein corresponding to full-length human 14-3-3 sigma

Application

Detect 14-3-3σ using this Anti-14-3-3σ Antibody, clone CS112-2A8 validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Recognizes 14-3-3σ, does not cross-react with other 14-3-3 isoforms.

Physical form

Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates of EGF-stimulated A431 cells

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	mouse
Quality Level	100
conjugate	unconjugated
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	CS112-2A8, monoclonal
species reactivity	mouse, human, rat
manufacturer/tradename	Upstate^®
technique(s)	immunoprecipitation (IP): suitable,western blot: suitable
isotype	IgG1κ
NCBI accession no.	NM_018754
UniProt accession no.	P31947
shipped in	dry ice
target post-translational modification	unmodified
Gene Information	human ... SFN(2810) mouse ... Sfn(55948) rat ... Sfn(313017)

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