Anti-MDM2 (Ab-2) Mouse mAb (2A10),100UG,OP115-100UGCN,Sigma

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订货号 0BV3586
品牌型号 Sigma OP115-100UGCN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Purified mouse monoclonal antibody (see application references). Recognizes the ~90 kDa (apparent MW) MDM2 protein.
Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells.
This Anti-MDM2 (Ab-2) Mouse mAb (2A10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-2).


Immunogen

Epitope: within amino acids 294-339
Human
human MDM2 protein


Application


Immunoblotting (2 g/ml)
Immunofluorescence (1 g/ml)
Immunoprecipitation (1 g/reaction, see application references)
Paraffin Sections (2.5 g/ml, heat pre-treatment required)


Packaging

Please refer to vial label for lot-specific concentration.


Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.


Analysis Note

Positive Control
A549 cells or most tissues


Other Notes

Antibody should be titrated for optimal results in individual systems.
Marchetti, A., et al. 1995. J. Pathol.175, 31.
Barak, Y., et al. 1993. EMBO. J.12, 461.
Chen, J., et al. 1993. Mol. Cell Biol.13, 4107.
Ladanyi, M., et al. 1993. Cancer Res.53, 16.
Leach, F.S., et al. 1993. Cancer Res.53, 2231.
Oliner, J.D., et al. 1993. Nature362, 857.
Momand, J., et al. 1992. Cell69, 1237.
Oliner, J.D., et al. 1992. Nature358, 80.
Fakharzadeh, S.S., et al. 1991. EMBO. J.10, 1565.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
biological sourcemouse
Quality Level100
antibody formpurified antibody
antibody product typeprimary antibodies
clone2A10, monoclonal
formliquid
contains≤0.1% sodium azide as preservative
species reactivityhuman
manufacturer/tradenameCalbiochem®
storage conditiondo not freeze
dilution (Immunoblotting (2 µg/mL)
Immunofluorescence (1 µg/mL)
Immunoprecipitation (1 µg/reaction,
Paraffin Sections (2.5 µg/mL, heat pre-treatment required))
isotypeIgG2a
shipped inwet ice
storage temp.2-8°C
target post-translational modificationunmodified
Gene Informationhuman ... MDM2(4193)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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