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General description
Ubiquitin conjugation factor E4 B (UniProt O95155; also known as Homologous to yeast UFD2, Homozygously deleted in neuroblastoma 1, Ubiquitin fusion degradation protein 2) is encoded by the UBE4B (also known as E4, HDNB1, UBOX3, UFD2, UFD2A) gene (Gene ID 10277) in human. The turnover of plasma membrane proteins involves internalization and sorting by the endocytic pathway. Ubiquitin modification allows proteins to be recognized by endosomally associated protein complexes, enabling sorting of proteins to be degraded from those to be recycled. The ubiquitin ligase UBE4B is reported to interact with "hepatocyte growth factor-regulated tyrosine kinase substrate" (Hrs) of the "endosomal sorting complex required for transport 0" (ESCRT-0) complex at the endosomal membrane, where UBE4B ubiquitinates internalized EGFR to allow its recognition by ESCRT-0. The deubiquinating enzyme USP8 then associates with ESCRT-0 via binding to "signal-transducing adaptor molecule" (STAM), while UBE4B dissociates from ESCRT-0. ESCRT-I and II then bind to cargo and facilitate membrane budding inward toward the lumen, whereas ESCRT-III mediates the dissociation of ESCRTs from endosomal membranes and membrane scission.
~130 kDa observed
Immunogen
Recombinant protein corresponding to human UBE4B.
Application
Detect UBE4B using this rabbit Polyclonal Anti-UBE4B Antibody, Cat. No. ABS1079, validated for use in Western Blotting and ICC.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Western Blotting Analysis: A representative lot detected recombinant UBE4B, endogenous UBE4B in STAM immunoprecipitate from HeLa cells, and siRNA-mediated UBE4B depletion in HeLa cells (Sirisaengtaksin, N., et al. (2014). J Biol Chem. 289(5):3026-3039).
Immunocytochemistry Analysis: A representative lot detected UBE4B cellular localization in HeLa cells with or without EGF-EGFR internalization (Sirisaengtaksin, N., et al. (2014). J Biol Chem. 289(5):3026-3039).
Biochem/physiol Actions
This antibody is expected to react with all 4 spliced isoforms of human UBE4B protein.
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing Tris-HCl with 0.05% sodium azide with 50% glycerol.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected UBE4B in 10 µg of K562 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_001099032 |
| UniProt accession no. | O95155 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UBE4B(10277) |
| 长度(mm) | |
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| 重量(kg) |
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