- 商品介绍
- 规格参数
- 包装参数
General description
The previously assigned protein identifier Q07814 has been merged into Q07812. Full details can be found on the UniProt database.
Immunogen
Epitope: N-terminus, aa3-16 hBax protein
Synthetic peptide corresponding to aa 3-16 (GSGEQPRGGGPTSS) of human bax. This amino acid sequence is not shared by mouse or rat Bax.
Application
Anti-Bax Antibody, N-terminus, aa3-16 hBax protein, clone 2D2 is an antibody against Bax for use in IF, WB, IH(P).
Research Category
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
Western blot: 1 μg/ml for 2 hours at RT (1:200 dilution)
Immunofluorescence
Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT)
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes a 21 kD protein. Does not cross-react with Bcl-2a or Bcl-X. In contrast to Bcl-2a and Bcl-X, overexpression of Bax protein accelerates apoptosis.
Physical form
Format: Purified
Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-434
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 2D2, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunofluorescence: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_004324.3,NM_138761.2,NM_138763.2,NM_138764.2,NM_138765.2 |
| UniProt accession no. | Q07812 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... BAX(581) |
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¥ 711.69
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