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General description
Caspase recruitment domain-containing protein 8 (UniProt: Q9Y2G2-5; EC:3.4.-.-; also known as CARD-inhibitor of NF-kappa-B-activating ligand (CARDINAL), Tumor up-regulated CARD-containing antagonist of CASP9 (TUCAN), CARD8-CT, CARD8-NT) is encoded by the CARD8 (also known as DACAR, KIAA0955, NDPP1) gene (Gene ID: 22900) in human. CARD8 is characterized by the presence of a CARD domain, which is crucial for protein-protein interactions, particularly in the formation of inflammasomes and signaling complexes. The protein also contains a FIIND (function to find domain) and a NOD (nucleotide-binding oligomerization domain), which are involved in self-cleavage and oligomerization, respectively. CARD8 primarily functions in the regulation of inflammatory responses and apoptosis. It acts as an inhibitor of NF- B, a key transcription factor in inflammatory processes, and plays a role in the formation of inflammasomes, which are essential for the activation of inflammatory caspases and the processing of pro-inflammatory cytokines like IL-1β. The expression of CARD8 is widespread but varies significantly among different tissues. It is predominantly found in immune cells and tissues with high metabolic activity, which are often sites of inflammation. Expression levels can be influenced by inflammatory stimuli and are subject to regulation by various cytokines and pathogen-associated molecular patterns. Dysregulation of CARD8 expression or function has been linked to conditions such as rheumatoid arthritis, Crohn′s disease, and gout, where aberrant inflammasome activity leads to excessive inflammation. Additionally, mutations or alterations in the CARD8 gene have been associated with increased susceptibility to these diseases. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 25 amino acids from the of C-terminal half of human CARD8.
Application
Quality Control Testing
Evaluated by Western Blotting in Human umbilical vein endothelial cells (HUVEC) lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CARD8 protein in Human umbilical vein endothelial cells (HUVEC) lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CARD8 in lysates from Jurkat cells and a 1:10,000 dilution detected CARD8 in lysates from SK-MEL-28 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CARD8 in human placenta tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CARD8 in human umbilical vein endothelial cells (HUVEC).
Affinity Binding Assay: A representative lot of this antibody bound CARD8 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1C23 is a ZooMAb® rabbit recombinant monoclonal antibody that detects CARD8. It targets an epitope within 25 amino acids from the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1C23, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 60.65 kDa,observed mol wt ~70 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgGκ |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_001171829 |
| UniProt accession no. | Q9Y2G2-5 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... CARD8(22900) |
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¥ 8293.41
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¥ 238.17
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¥ 4404.38
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¥ 8982.64
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