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General description
FAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src (Guan, J L and Shalloway, D). This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton. Additional components of focal adhesions include actin, filamin, vinculin, talin, paxillin and tensin (Burridge, K, et al).
~125 kDa
Immunogen
Epitope: Terminal
Recombinant fragment derived from the amino terminal region of chicken FAK, amino acid residues 103 to 553.
Application
Immunohistochemistry Analysis: A previous lot detected Focal Adhesion Kinase at 1:100 dilution in mouse cortex tissue.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
This Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7 is validated for use in WB, IP for the detection of Focal Adhesion Kinase.
Biochem/physiol Actions
This antibody recognizes Focal Adhesion Kinase
Physical form
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HEK293 cell lysate
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Focal Adhesion Kinase in 10 µg of HEK293 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-591
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | BLAb2H7, monoclonal |
| species reactivity | human, mouse, rat, chicken |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_990766.1 |
| UniProt accession no. | Q05397 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | chicken ... Ptk2(396416) human ... PTK2(5747) mouse ... Ptk2(14083) rat ... Ptk2(25614) |
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