Anti-Synaptopodin (SE-19) antibody produced in rabbit，200UL，S9442-200UL，Sigma

General description

Synaptopodin is a proline-rich, actin-associated protein (100 kDa) is present in the post-synaptic density (PSD) in actin cytoskeleton. It is also present in the dendritic spines and differentiated podocytes. Synaptopodin contains a high amount of proline residues (~20%), which are evenly distributed along the protein sequence, thus virtually excluding the formation of globular domains. In particular, synaptopodin does not contain functional domains found in receptor clustering PSD proteins. Sequence comparison between human and mouse synaptopodin shows 84% identity. The exclusive site of synaptopodin expression in the telencephalon is restricted to specific areas of high synaptic plasticity, i.e. in the perykaria of the olfactory bulb, striatum, and hippocampus.

Immunogen

synthetic peptide corresponding to amino acids 184-202 of rat synaptopodin conjugated to KLH. This rat synaptopodin sequence has ~70% identity with human synaptopodin.

Application

Anti-Synaptopodin (SE-19) antibody produced in rabbit has been used in:

• immunofluorescence microscopy
• immunohistochemistry
• western blotting

Biochem/physiol Actions

Synaptopodin is thought to play an important role in synaptic plasticity and is associated in the modulation of motility of actin in neuronal dendritic spines. The association of synaptopodin with the specialized actin system of renal podocyte foot processes suggests that it may play a role in actin cytoskeleton dynamics of these cellular extensions. Synaptopodin has been found to interact with α-actinin-4 and the tight junction protein Membrane-associated guanylate kinase inverted 1 (MAGI-1) in Madin-Darby Canine Kidney (MDCK) cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.