MULTI-seq Lipid-Modified Oligos，100REACTIONS，LMO001-100RXN，Sigma

General description

MULTI-seq, multiplexing using lipid-tagged indices (lipid-modified oligonucleotides), is a novel tool that facilitates multiplex single-cell and single-nucleus RNA sequencing. This technology provides sample barcoding that is compatible with any cell type or nucleus containing an accessible plasma membrane and allows for streamlined, pooled single-cell sample processing. Learn more about the experiments and data by reviewing our technical article – (MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing)

Features and Benefits

• Efficient. MULTI-seq increases the throughput and decreases the reagent cost of droplet-based single-cell RNA sequencing.
• Flexible. Compatible with any droplet generation platform, the indexing system enables users to run and analyze up to 96 barcoded samples simultaneously in an 8-lane droplet device.
• Clearer Results. The indices provide data quality advantages over non-indexed analysis methods in the form of doublet identification and retention of data from cells with low RNA content.

Other Notes

• This product is for R&D use only. Not for drug, household, or other uses.
• Unique barcode oligos are not included and must be purchased separately.
• Barcode 3’ oligo design with poly-A tail for mRNA enrichment: 5′-CCTTGGCACCCGAGAATTCCA-8-base index-A30-3′
• Barcode oligo design for 5’ cDNA library synthesis: 5’-CCTTGGCACCCGAGAATTCCA-8-base indexCCCATATAAGAAA-3’
• In addition, 96 x 5′-end and 96 x 3′-end unique barcode oligos are available to purchase separately. Details on our custom barcode product, Next-Gen Sequencing Oligos (NGSO), can be found at SigmaAldrich.com/nextgenoligos. For minimal cross-contamination of barcode primers, order NGSO-Silver or preferably, NGSO-Gold quality. Please review the specifications available and then submit a quote request for the MULTI-seq barcodes to dnaoligos@milliporesigma.com. Sequences are not provided prior to purchase but will be included on product documentation at the time of delivery.
  

• LMO001A Lignoceric Anchor with DNA Oligo
• LMO001B Palmitic Co-anchor with DNA Oligo