- 商品介绍
- 规格参数
- 包装参数
General description
Glycoprotein (UniProt: P03524) is encoded by the G gene in Rabies virus. The glycoprotein (G) of the Evelyn Rokitniki Abelseth (ERA) strain of the rabies virus is a single-pass type I membrane protein that plays a critical role in the virus′s ability to infect host cells. It is composed of a single polypeptide chain, featuring an ectodomain, a transmembrane domain, and a short cytoplasmic tail. The ectodomain contains multiple glycosylation sites, which contribute to its stability and immunogenicity. The primary function of the ERA glycoprotein is to mediate viral entry into host cells by binding to nicotinic acetylcholine receptors (nAChRs) on neuronal cells. It facilitates the fusion of the viral envelope with the host cell membrane, allowing the viral genome to enter the host cell. The glycoprotein is crucial for eliciting an immune response. The glycoprotein is expressed during the viral replication cycle, primarily in infected neurons and other susceptible cells. Its expression is regulated by the rabies virus′s life cycle, peaking during the assembly and budding phases of viral replication. The glycoprotein is localized at the plasma membrane of infected cells, where it is incorporated into new virions. As a key component of the rabies virus, the ERA glycoprotein is directly involved in the pathogenesis of rabies. Infection leads to severe neurological symptoms and is often fatal if not treated promptly. The glycoprotein′s immunogenic properties make it a critical target for rabies vaccines, which aim to elicit an effective immune response against the virus. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
Purified Glycoprotein of ERA (Evelyn Rokitniki Abelseth) strain of Rabies virus.
Application
Quality Control Testing
Evaluated by Immunocytochemistry in L929 cells transfected with Rabies Virus Glycoprotein.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected RABV Glycoprotein in L929 cells stably transfected with glycoprotein of ERA strain of Rabies virus.
Tested Applications
Flow Cytometry Analysis: 1 µg from a representative lot detected RABV Glycoprotein in L929 cells stably transfected with glycoprotein of ERA strain of Rabies virus.
Neutralizing Assay: A representative lot neutralized Rabies Virus (Bunschoten, H., et. al. (1989). J Gen Virol. 70 ( Pt 2):291-8; Dietzchold, B., et. al. (1992). Proc Natl Acad Sci USA. 89(15):7252-6; Muller, T., et. al. (2009). PLoS Neql Trop Dis. 3(11):e542; Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5).
ELISA Analysis: A representative lot of this antibody detected Rabies Virus (Morgeaux S., et al. (2017). 35(6); 966-971)
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone1112-1 is a ZooMAb® mouse recombinant monoclonal antibody that detects RABV Glycoprotein. It targets an epitope within the Glycoprotein of ERA (Evelyn Rokitniki Abelseth) strain Rabies virus.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1112-1, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 58.66 kDa,observed mol wt ~N/A kDa |
| purified by | using Protein A |
| species reactivity | rabies virus |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,flow cytometry: suitable,immunocytochemistry: suitable,neutralization: suitable |
| isotype | IgG2bκ |
| epitope sequence | Unknown |
| Protein ID accession no. | N/A |
| UniProt accession no. | P03524 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
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