- 商品介绍
- 规格参数
- 包装参数
General description
Manufactured for Sigma by Boston Biochem., Inc.
Application
Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.
Biochem/physiol Actions
Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.
Protein fraction of a cell extract that binds to anion-exchange resin.
Physical form
Solution in 25 mM HEPES, pH 7.6.
| Quality Segment | 200 |
| form | solution |
| concentration | 5 mg/mL |
| storage temp. | −70°C |
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¥ 1596.77
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¥ 1779.22
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¥ 3372.68
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¥ 2617.87
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¥ 1564.00
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¥ 1749.15
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¥ 1156.91
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¥ 8137.12
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