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General description
Src is a proto-oncogene that regulates neuronal functions, such as nerve cell differentiation. The Src kinase family structurally comprises of SH2, SH3 and kinase domains, as well as a negative regulatory C-terminal tail. Src has also been implicated in functions such as cell growth, development and cell-cell interactions . Anti-phospho-Src p(Tyr418) antibody is specific for human, mouse, rat and chicken Src (60 kDa).
Immunogen
The sequence is conserved in human, mouse, chicken and frog.
synthetic phosphopeptide derived from the region of src that contains tyrosine 418, based on the sequence of human src.
Application
Anti-phospho-Src p(Tyr418) antibody is suitable for use in western blot (1:1,000 using human platelets or mouse NIH3T3 cells). The product may also be used for immunoprecipitation (1:1000 using HEK293 or HEL cell lysates ).
Physical form
Solution in Dulbecco′s phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/ml BSA (IgG and protease free) and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous glycerol solution |
| species reactivity | chicken, mouse, human, rat |
| technique(s) | western blot: 1:1,000 using human platelets or mouse NIH3T3 cells |
| UniProt accession no. | P12931 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr418) |
| Gene Information | human ... SRC(6714) mouse ... Src(20779) rat ... Src(83805) |
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