Anti-FOXL2 antibody produced in rabbit，100UG，F0805-100UG，Sigma

General description

FOXL2 encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3.

Immunogen

synthetic peptide M(1)MASYPEPEDTAGT(14) corresponding to amino acid residues 1-14 from mouse FOXL2. This sequence is completely conserved in mouse and rat and 92% conserved in human.

Application

Anti-FOXL2 antibody produced in rabbit is suitable for the following applications:

• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 0.2μg/mL using murine prenatal folicle
• Indirect immunofluorescence at a concentration of 2μg/mL using KK1 granulosa cells
• Western blotting (at a concentration of 0.2μg/mL using CHO whole cell lysate) or immunoblot analysis.

Biochem/physiol Actions

Forkhead box protein L2 is a protein encoded by the FOXL2 gene in humans. The gene FOXL2 encodes a member of the fork-head-winged-helix family of transcription factors. It is expressed early during female gonadal development and is expressed in normal ovarian stroma and ovarian neoplasms with granulosa cell lineage. FOXL gene encodes an essential transcription factor in the ovary and helps in female sex determination, follicle recruitment and granulosa cell development. Its mutation is specific to AGCTs in the ovary and is useful for diagnosis of disease. FOXL2 acts as an oncogene or tumour suppressor depending on the genetic context that is the GCT subtype.

Physical form

Provided as 100 μg of affinity purified IgG (1 mg/mL) in phosphate buffered saline containing 1 mg/mL bovine serum albumin and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.