- 商品介绍
- 规格参数
- 包装参数
General description
The Lenti CRISPR Non-Target Control#2 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#2 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Control#2 uses a dual-component system consisting of U6-driven guide RNA (non-target#2) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Application
Functional Genomics/Screening/Target Validation
Other Notes
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.
Legal Information
CRISPR Use License Agreement
Lentiviral, WPRE, and Evrogen Licenses
| form | liquid |
| Quality Segment | 200 |
| packaging | vial of 200 μL |
| concentration | (1x106 TU/mL (via p24 titering assay)) |
| application(s) | CRISPR |
| shipped in | dry ice |
| storage temp. | −70°C |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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¥ 5887.85
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¥ 8520.40
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¥ 4390.38
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¥ 4404.38
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¥ 2151.46
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¥ 745.60
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¥ 5061.38
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¥ 1586.69
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¥ 2132.12
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¥ 10685.48
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¥ 3796.67
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¥ 165230.53
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