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General description
B-cell lymphoma/leukemia 11B (UniProt: Q9C0K0; also known as BCL-11B, B-cell CLL/lymphoma 11B, COUP-TF-interacting protein 2, Radiation-induced tumor suppressor gene 1 protein, hRit1) is encoded by the BCL11B (also known as CTIP2, RIT1) gene (Gene ID: 64919) in human. Bcl-11B is a zinc finger protein transcription factor plays a critical role in the development, proliferation, differentiation, and survival of T cells. It is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. Bcl-11B works as both a genetic suppressor and activator, acting directly, attaching to promoter regions, as well as indirectly, attaching to promoter-bound transcription factors. It contains six C2H2-type zinc finger domains. Of those, domains 3 and 4 bind to the DNA, while other regions are important for protein interactions. It is shown to be a key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals. It also plays a role in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus. Bcl-11B also acts as a tumor suppressor that represses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. In a murine model. The loss of a BCL11B gene increases susceptibility to thymic lymphoma in murine models and to acute lymphoblastic leukemia (T-ALL) in human. Bcl-11B also plays a role in fetal neuronal development, with important roles for the differentiation and development of various neuronal subtypes in the central nervous system. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lennon, MJ., et al. (2017). Front. Cell. Neurosci. 11; 89; Huang, X., et al. (2012). Exp. Hematol. Oncol. 1; Article 22).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated liner peptide corresponding to 16 amino acids from the internal region of human B-cell lymphoma/leukemia 11B (Bcl-11B).
Application
Quality Control Testing
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Bcl-11B in Jurkat cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Bcl-11B in MOLT4 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bcl-11B in Jurkat cells.
Affinity Binding Assay: A representative lot of this antibody bound Bcl-11B with a KD of 1.3 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Bcl-11B in Jurkat cell lysate.
Biochem/physiol Actions
Clone 1G14 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects B-cell lymphoma/leukemia 11B (Bcl-11B). It targets an epitope within 16 amino acids from the internal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1G14, recombinant monoclonal |
| description | 1G14 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 95.52 kDa,observed mol wt ~110 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine, horse, mouse, rat, monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Internal |
| Protein ID accession no. | NP_612808 |
| UniProt accession no. | Q9C0K0 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... BCL11B(64919) |
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