- 商品介绍
- 规格参数
- 包装参数
General description
TargeTron™ vector pJIR750ai is a 10,262 bp expression vector intended for use with the TargeTron Gene Knockout System, Catalog Number TA0100. This circularized vector can be used for targeted gene knockouts in gram-positive bacteria such as Clostridium perfringens type A.1 Expression of the group II intron RNA is under the control of the β-2 toxin gene promoter, cpb2.1 As supplied, the pJIR750ai vector is re-targeted to the α-toxin (plc) gene. α-Toxin, a phospholipase C, is an extracellular toxin produced by all C. perfringens isolates.1 For validation in a specific C. perfringens strain, this vector can be used directly to knockout plc without any further modifications using the enclosed protocol. In order to re-target this vector to knockout other genes, the plc specific IBS-EBS fragment (350 bp) between the Hind III and BsrG I sites can be cut out and replaced with another gene specific fragment. For re-targeting protocols, refer to the TargeTron Gene Knockout System User Guide, Catalog Number TA0100, at sigma-aldrich.com.
Legal Information
TargeTron is a trademark of InGex, LLC
| Quality Segment | 200 |
| storage temp. | −20°C |
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¥ 1302.89
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瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmPES滤膜，孔径0.45um,适合瓶颈尺寸33mm，12/箱，295-3345，Nalgene，Thermofisher，赛默飞世尔¥ 1938.54
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¥ 1476.00
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¥ 1586.69
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¥ 2277.64
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¥ 2754.43
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¥ 56581.65
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¥ 44224.19
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