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- 规格参数
- 包装参数
General description
Volume-regulated anion channel subunit LRRC8E (UniProt: Q6NSJ5; also known as Leucine-rich repeat-containing protein 8E) is encoded by the LRRC8E gene (Gene ID: 80131) in human. Leucine-rich repeat-containing protein 8E (LRRC8E) belongs to the leucine-rich repeat-containing family, characterized by extracellular leucine-rich repeats that facilitate protein-protein interactions. LRRC8E functions as a subunit of the volume-regulated anion channel (VRAC), which is essential for regulating cell size and volume homeostasis by transporting ions and organic molecules across cellular membranes. VRAC complexes typically consist of LRRC8A as the core subunit and other subunits such as LRRC8B, LRRC8C, LRRC8D, and LRRC8E, which modulate channel specificity and function. LRRC8E exhibits expression in various tissues, with high levels in the lung and spleen. It contributes to the transport of cyclic GMP-AMP (cGAMP), a secondary messenger involved in immune activation, highlighting its role in immune signaling pathways. LRRC8E is localized to the cell membrane and lysosomes, where it participates in lysosomal homeostasis under specific conditions. While LRRC8E is not essential for VRAC function, its presence diversifies the channel′s activity, influencing cellular responses to osmotic stress and apoptotic signaling. LRRC8E has been implicated in diseases such as long QT syndrome and megalencephalic leukoencephalopathy with subcortical cysts. Recent studies suggest potential associations of LRRC8E with psychiatric disorders, including panic disorder, although further research is needed to establish its role in these conditions. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal cytoplasmic domain of human LRRC8E.
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRRC8E in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRRC8E in lysate from wild-type HeLa cells, but not in lysate from LRRC8A/B/C/D/E knockout HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1E10 is a ZooMAb® rabbit recombinant monoclonal antibody that detects LRRC8E. It targets an epitope within 18 amino acids from the C-terminal cytoplasmic domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 90.25 kDa,observed mol wt ~75/80 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal cytoplasmic domain |
| Protein ID accession no. | NP_001255213 |
| UniProt accession no. | Q6NSJ5 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... LRRC8E(80131) |
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