FastPlax Titer Kit - Novagen，5EA，70850-3，Sigma

General description

The FastPlax Titer Kit is designed for determination of baculovirus titers in just 24 to 48 hours instead of three to four days as in other methods. Classical determination of baculovirus titer has relied on the appearance of plaques, but the FastPlax Kit takes advantage of the appearance of detectable levels of AcNPV gp64 glycoprotein on the cell surface as early as 8 to 24 hours postinfection. Detection is based on a high affinity monoclonal antibody against gp64, which is added directly to fixed cells. Antibody binding is detected with Goat Anti-Mouse IgG β-Galactosidase Conjugate followed by enhanced color development with X-Gal/NBT substrates. Plaques are clearly distinguished by their dark blue color. The kit contains sufficient reagents to perform five 6-well plate assays.

Packaging

Plastic ampoule

Preparation Note

Multiple storage requirements

Other Notes

• 2 × 50 ml- 10X TBST (100 mM Tris-HCl, pH 8.0, 1.5 M NaCl, 1% Tween^® 20)
• 6 ml- 10% Gelatin (contains 0.02% azide)
• 10 µl- FastPlax Antibody
• 300 µl- Goat-Anti-Mouse β-Galactosidase Conjugate
• 300 µl- NBT (83 mg/ml in 70% dimethylformamide)
• 300 µl- X-gal (50 mg/ml in dimethylformamide)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS