- 商品介绍
- 规格参数
- 包装参数
General description
Monoclonal Anti-USP8 (mouse IgG1 isotype) is derived from the hybridoma US872 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Ubiquitin specific protease 8 (USP8) belongs to the family of de-ubiquitinating enzymes (DUBs).
Immunogen
synthetic peptide corresponding to an internal sequence of human USP8, conjugated to KLH.
Application
Monoclonal Anti-USP8 antibody produced in mouse has been used in:immunoblotting immunofluorescence,flow cytometry, immunocytochemistry.
Biochem/physiol Actions
Ubiquitin specific protease 8 (USP8) was found to regulate ubiquitination of frizzled, a receptor for Wnt/Wingless. Deletion of USP8 in mice causes embryonic lethality. The defect was accompanied by a strong reduction or absence of several growth factor receptor tyrosine kinases (RTKs), like EGFR, hepatocyte growth factor receptor (c-met), and ERBB3.USP8 inactivation results in enhanced ubiquitination of ligand-activated EGFR. USP8 in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect epidermal growth factor receptor (EGFR) as well as other receptors from lysosomal degradation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | US872, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~127 kDa,antigen ~70 kDa |
| species reactivity | human, rat, bovine, monkey, mouse, dog |
| concentration | ~1.0 mg/mL |
| technique(s) | flow cytometry: 5-10 μg/test using MCF7 cells,indirect immunofluorescence: 1.25-2.5 μg/mL using A549 cells,western blot: 0.5-1.0 μg/mL using MCF7 total cell extracts |
| UniProt accession no. | P40818 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... USP8(9101) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 1302.89
-
¥ 1451.54
-
¥ 3109.49
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 6330.00
-
瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmSFCA滤膜，孔径0.2um,适合瓶颈尺寸45mm，12/箱，291-4520，Nalgene，Thermofisher，赛默飞世尔¥ 2856.42
-
¥ 3319.60
-
¥ 2307.00
-
¥ 1509.00
-
¥ 1301.36
-
¥ 1476.40
-
¥ 3031.24
-
¥ 3476.87
-
¥ 3662.39
-
¥ 56581.65
-
询价
微信小程序
7X24小时在线咨询