- 商品介绍
- 规格参数
- 包装参数
General description
Interleukin-2 receptor subunit beta (UniProt: P14784; also known as IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, p70-75 (p75), CD122) is encoded by the IL2RB (also known as IL15RB) gene (Gene ID: 3560) in human. IL-2R is a non-covalent dimer of an alpha and a beta subunit. It exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. IL2RB is a single-pass type I membrane protein. It is synthesized with a signal peptide (aa 1-26), which is cleaved off to produce the mature form that has an extracellular domain (aa 27-240), a transmembrane domain (aa 241-265), and a long cytoplasmic domain (aa 266-551). IL2RB contains a WSXWS motif (aa 220-224), which is essential for its proper folding and thereby efficient intracellular transport and cell-surface receptor binding. The protein also has a Box 1 motif (aa 278-286) that is required for JAK interaction and/or activation. This receptor is predominantly expressed in T cells, but it is also found in other immune cells, including B cells and natural killer cells. Dysregulation of IL2RB expression or function has been implicated in several diseases, including autoimmune disorders and cancer. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhang, Z., et al. (2019). J. Exp. Med. 216(6);1311 1327); Y, Liao., et al. (2022). BioMed. Res. Int. 2022:2114699).
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal, extracellular domain of human IL2RB.
Application
Quality Control Testing
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IL2RB in Raji cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IL2RB in lysates from HEK-293T cells and rat spleen tissue.
Affinity Binding Assay: A representative lot of this antibody bound IL2RB peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected IL2RB in human spleen tissue and human bone marrow tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 4D2 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects IL2RB. It targets an epitope within 22 amino acids from the N-terminal, extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Other Notes
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4D2, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 61.11 kDa,observed mol wt ~68 kDa |
| purified by | using Protein A |
| species reactivity | human, rat |
| species reactivity (predicted by homology) | chimpanzee, monkey |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal extracellular domain |
| Protein ID accession no. | NP_000869 |
| UniProt accession no. | P14784 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... IL2RB(3560) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
询价
-
¥ 2290.21
-
¥ 1779.22
-
¥ 5887.85
-
询价
-
询价
-
询价
-
¥ 238.17
-
询价
-
询价
-
询价
-
¥ 6330.00
-
询价
-
¥ 4020.77
-
¥ 2277.64
-
¥ 1351.48
-
¥ 10685.48
-
NNALGENE 180透明塑料管，透明，柔软，多用，经济，内径 x 外径x壁厚，1/4 x 1/2 x 1/8，英寸，每箱数量50，50/箱，8000-0080，Thermofisher，赛默飞世尔询价
-
¥ 5426.00
微信小程序
7X24小时在线咨询