Anti-MBP Magnetic Beads，1ML，SAE0218-1ML，Sigma

General description

Anti-MBP Magnetic Beads are a premium solution for researchers seeking to purify MBP-tagged fusion proteins. Designed for efficiency and reliability, these magnetic beads utilize a high-affinity anti-Maltose Binding Protein (MBP) antibody, ensuring optimal binding and elution of your target proteins. With a binding capacity of 20-62 nmole per mL of settled resin, this product is ideal for applications in molecular biology, biochemistry, and protein research. The Anti-MBP Magnetic Beads are essential for anyone working with recombinant proteins, enabling precise isolation and purification under mild conditions.

Application

The Anti-MBP Magnetic Beads are specifically engineered for the purification of MBP fusion proteins in bacterial systems or mammalian cells transfected with MBP expression vectors. This product is particularly advantageous for researchers dealing with low-abundance proteins or those that are poorly immunogenic. By using these magnetic beads, you can efficiently capture MBP-tagged proteins from complex biological samples, facilitating downstream applications such as enzyme assays, structural studies, and functional analyses.

Features and Benefits

• High Binding Capacity: Achieve superior results with a binding capacity of 20-62 nmole of MBP-tagged fusion protein per mL of settled resin.
• Versatile Applications: Suitable for use in bacterial extracts and transfected mammalian cells, making it a versatile choice for various research settings.
• Stable Fusion Product: The MBP tag creates a stable fusion product that does not interfere with the bioactivity of the protein, ensuring reliable results.
• Easy Purification: Simplify your workflow with a single-step affinity purification process on amylose resin, allowing for rapid and efficient protein isolation.

Preparation Note

For optimal performance and longevity, store the Anti-MBP Magnetic Beads at 2-8 °C. It is crucial to avoid freezing, as this may compromise the integrity of the beads. Before use, ensure thorough mixing of the beads by gentle vortexing or inversion to maintain homogeneity. These precautions will help preserve the binding capacity and overall effectiveness of the product over time.