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General description
当使用MISSION® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Transduction Particles are a positive control to monitor transduction efficiency for applications in which an alternate promoter is necessary. The MISSION UbC-TurboGFP Control Transduction Particles are produced from the sequence-verified 9092 base pair lentivirus plasmid, pLKO.1-puro-UbC-TurboGFP (SHC014).
This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
| Quality Segment | 100 |
| product line | MISSION® |
| concentration | ≥1x106 VP/ml (via p24 assay) |
| technique(s) | capture ELISA: 106 TU/mL using p24 |
| shipped in | dry ice |
| storage temp. | −70°C |
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¥ 8135.09
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