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General description
Application
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.
Biochem/physiol Actions
Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.
L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.
谷氨酸降解酶
Physical form
Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts
Other Notes
One unit will convert 1.0 μmole of
| form | lyophilized powder |
| Quality Segment | 200 |
| specific activity | 400-2,000 units/mg protein |
| purified by | affinity chromatography |
| contains | dithioerythritol as preservative |
| composition | Protein, ~5% Lowry |
| solubility | H2O: soluble 0.95-1.05 mg/mL, clear to hazy |
| UniProt accession no. | P0A9C5 |
| foreign activity | ATPase <0.2% |
| storage temp. | −20°C |
| Gene Information | Escherichia coli K12 ... glnA(948370) |
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¥ 2013.69
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NNALGENE 180透明塑料管，透明，柔软，多用，经济，内径 x 外径x壁厚，1/4 x 1/2 x 1/8，英寸，每箱数量50，50/箱，8000-0080，Thermofisher，赛默飞世尔询价
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