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Biochem/physiol Actions
SARS-CoV and SARS-CoV-2 main protease (Mpro; 3CLpro) fluorescence energy transfer (FRET) substrate with >10-fold sensitivity than Edans-Dabcyl-based substrate.
Abz-SVTLQSG-TyrNO2R (Abz = 2-aminobenzoyl; TyrNO2 = 2-nitro-L-tyrosyl) is a substrate for the detection of SARS-CoV and SARS-CoV-2 main protease (Mpro; 3CLpro) activity by fluorescence energy transfer (FRET) with >10-fold sensitivity than the corresponding Edans-Dabcyl-based FRET peptide. Substrate cleavage by Mpro between Gln and Ser leads to increased fluorescence (ex 330+/-10 nm, em 420+/-20 nm) as a result of the separation of the N-terminal Abz (fluorescent donor) and the C-terminal TyrNO2 (fluorescent quencher).
Disclaimer
Hygroscopic
| Quality Segment | 100 |
| assay | ≥95% (HPLC) |
| form | (Powder or Lyophilized powder or film) |
| storage condition | desiccated |
| color | , White to light brown |
| storage temp. | -10 to -25°C |
| SMILES string | N=C(NCCC[C@H](NC([C@H](CC1=C(C=C(O)C=C1)[N+]([O-])=O)NC(CNC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC(C2=C(N)C=CC=C2)=O)CO)=O)C(C)C)=O)[C@@H](C)O)=O)CC(C)C)=O)CCC(N)=O)=O)CO)=O)=O)=O)C(O)=O)N |
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¥ 5167.58
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