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General description
Anti-rabbit IgG (whole molecule) is produced in goat using purified rabbit IgG as the immunogen. Antibody is isolated from anti-rabbit IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins that do not specifically bind to rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Anti-Rabbit IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
Specificity of the antiserum is determined by immunoelectrophoresis prior to conjugation, versus normal rabbit serum and rabbit IgG. Identity and purity of the antibody is established by immunoelectrophoresis prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity (Fab fragments). The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc).
Immunogen
Purified rabbit IgG
Application
通过 ELISA 法分析 PADI4 对抗凝血酶的瓜氨酸化作用，使用碱性磷酸酶标记的羊抗兔 IgG 作为在0.05M碳酸盐/碳酸氢盐缓冲液（Ph 9.6）中以1：5000稀释的二次稀释剂。
Goat anti-rabbit IgG (whole molecule)-alkaline phosphatase antibody can be used for western blot and ELISA
It can also be used for immunohistochemistry (1:50) and dot bot (1:30,000) assays.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide
Preparation Note
Adsorbed to reduce background staining with human samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Segment | 200 |
| conjugate | alkaline phosphatase conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous glycerol solution |
| species reactivity | rabbit |
| technique(s) | direct ELISA: 1:30,000,dot blot: 1:30,000,immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50,western blot: 1:30,000 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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