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General description
Ribonucleoside-diphosphate reductase large subunit (UniProt: P23921; also known as EC:1.17.4.1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large subunit, RRM1) is encoded by the RRM1 (also known as RR1) gene (Gene ID: 6240) in human. RRM1 is a member of the ribonucleoside diphosphate reductase large chain family. Ribonucleotide reductase (RNR) catalyzes the rate-limiting step of de novo nucleotide synthesis through reduction of ADP, CDP, GDP, and UDP to their deoxynucleotide equivalents. The RNR complex consists of catalytic RRM1 dimers in complex with a smaller subunit RRM2. Two distinct regulatory sites have been defined for RRM1: the specificity site that controls substrate specificity; and the activity site that regulates its overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2. RRM1 is functionally associated with DNA replication and DNA damage repair. Level of its enzyme activity is closely correlated with the growth rate of a cell and appears to vary with the cell cycle. Higher expression of RRM1 has been linked with poor clinical outcome and poor overall survival on adjuvant chemotherapy in patients with pancreatic cancer. Cancer cells lacking cytoplasmic RRM1 activation exhibit severe DNA damage. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shintaku, J., et al. (2022). J. Clin. Invest. 132(13):e145660; Kato, T., et al. (2021). PLoS ONE 16(6); e0252917; Bepler, G., et al. (2006). J. Clin. Oncol. 24(29); 4731-4737).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human Ribonucleotide reductase large subunit (RRM1).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RRM1 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RRM1 in A549 and COS-7 cell lysates.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected RRM1 in one million HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RRM1 in human tonsil tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound RRM1 with an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1E20 is ZooMAb® rabbit recombinant monoclonal antibody that specifically detects RRM1. It targets an epitope within 22 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1E20, recombinant monoclonal |
| description | 1E20 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 90.07 kDa,observed mol wt ~80 kDa |
| purified by | using Protein A |
| species reactivity | human, monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_001024 |
| UniProt accession no. | P23921 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... RRM1(6240) |
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