FXR, ligand binding domain (222-472), GST tagged human，10UG，SRP2137-10UG，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

Biochem/physiol Actions

Farnesoid-X-activated receptor (FXR) was originally identified and cloned in rat as an orphan nuclear hormone receptor based on hybridization with a degenerate oligonucleotide designed from the highly conserved nuclear hormone receptor DNA binding domain. FXR functions as a heterodimer with RXR and binds to sequence elements in the promoters of target genes. The FXR/RXR heterodimer binds with highest affinity to inverted repeats separated by 1 bp (IR-1) and with low affinity to direct repeats separated by 4 and 5 bp (DR-4 and DR-5). As is the case for other nuclear hormone receptors, FXR regulates target gene activity in response to ligand. While initial studies suggested that farnesol and retinoid metabolites were likely ligands for FXR, current data support the notion that FXR is a bile acid sensor that plays an integral role in bile acid synthesis and transport. In the small intestine, FXR regulates bile acid uptake through the upregulation of the ileal bile acid binding protein gene via binding to an upstream response element. The FXR/RXR heterodimer can be activated by the bile salt chenodeoxycholic acid (CDCA) and FXR is required for the bile salt-dependent transcriptional control of the human ABCB11 gene (the bile salt export pump). In addition, FXR has been shown to inhibit the cholesterol 7-hydrolase gene (CYP7A1) transcription.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

技术参数 Specifications

biological source	human
recombinant	expressed in E. coli
assay	≥70% (SDS-PAGE)
form	frozen liquid
mol wt	~56.3 kDa
packaging	pkg of 10 μg
storage condition	avoid repeated freeze/thaw cycles
concentration	400 μg/mL
color	colorless to clear
NCBI accession no.	U68233
UniProt accession no.	Q96RI1
shipped in	dry ice
storage temp.	−70°C
Gene Information	human ... HRR-1(9971)

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