抗-TJP1 兔抗，100UL，HPA001637-100UL，Sigma

General description

Tight junction protein 1/Zona occludens 1 protein (TJP1, ZO-1) is a transmembrane plaque protein belonging to the membrane-associated guanylate kinase (MAGUK) family of scaffold proteins. It consists of three PDZ (PSD-95/Discs large/ZO-1) domains, one SH3 (Src-homology 3) domain, and one GK (guanylate kinase) domain. Additionaly, it also contains a long proline-rich carboxyl terminal region and a ZU5 domain.

Immunogen

Tight junction protein ZO-1 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

Tight junction protein ZO-1 is a protein encoded by the TJP1 gene in humans and is mapped to chromosome 15q13. It is a membrane-scaffolding protein that plays an important role in maintaining tight-junction integrity, which is disrupted in many invasive cancers and intestinal diseases. It may act as a tumor-suppressor gene in GIST. It is an integral component of functional TJs and is regulated by p38δ. It shows a hypermethylation status in children with non-Hodgkin lymphoma (NHL) and the methylation level is positively correlated with the number of malignant cells in bone marrow. It may be useful as a novel molecular marker in early diagnosis, outcome assessment, prognostic evaluation and detection of minimal residual disease.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Other Notes

Corresponding Antigen APREST83051

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.