磷酸酶,碱性虾,500UNITS,A2237-500UN,Sigma

销售价: ¥ 2764.41 / 件
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订货号 0BM2073
品牌型号 Sigma A2237-500UN
货期 询货期
最小订货量 1件
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  • 商品介绍
  • 规格参数
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产品介绍 Product Description

General description

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.


Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.


Biochem/physiol Actions

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.


Physical form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2


Analysis Note

Protein determined by biuret


Other Notes

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.


技术参数 Specifications
recombinantexpressed in proprietary host
Quality Level200
formbuffered aqueous glycerol solution
concentration≥900 DEA units/mL
foreign activityRNAse, DNAse, none detected
shipped indry ice
storage temp.−20°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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