- 商品介绍
- 规格参数
- 包装参数
General description
Protein tyrosine phosphatase, non-receptor type 11 (PTPN11), also known as SHP-2, is a scaffolding protein. It possesses two SH2 domains at the amino terminal, a protein tyrosine phosphatase domain and carboxy-terminal region. The gene encoding SHP-2 is localized on human chromosome 12q24.13.
Application
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Human active SHP-2 has been used to study the effects of α-lipoic acid and dihydrolipoic acid on the activity of SHP-2 enzyme.
Biochem/physiol Actions
Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) or SHP-2 is involved in signal transduction pathways downstream of growth receptors. It acts as a proto-oncogene. SHP-2 leads to sustained RAS activation by dephosphorylating the RAS-GTPase-activating protein binding site on Grb2-associated binder 1 (Gab1). This protein also nucleates multiple signaling proteins and thus, acts as an adaptor protein. Mutations in the SHP-2 gene have been associated with Noonan syndrome.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
Amino acids : 224-529
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥90% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 59.5 kDa |
| packaging | pkg of 20 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | 0.73 mg/mL |
| NCBI accession no. | NM_002834 |
| UniProt accession no. | Q06124 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PTPN11(5781) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
¥ 2290.21
-
¥ 1573.49
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 3290.05
-
¥ 4404.38
-
¥ 2617.87
-
¥ 745.60
-
¥ 2173.94
-
询价
-
¥ 200318.99
-
¥ 57595.03
-
¥ 1799.83
-
询价
-
询价
-
询价
微信小程序
7X24小时在线咨询