Anti-Ran antibody,Mouse monoclonal，200UL，R4777-200UL，Sigma

General description

Monoclonal Anti-Ran (mouse IgG2b isotype) is derived from the ARAN1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BDF1 mice immunized with denatured recombinant human Ran. Ran is a small guanine trinucleotide phosphatase (GTPase) of the Ras superfamily that like other GTPases exist in two states: bound to guanine trinucleotide phosphate (GTP) or to guanine dinucleotide phosphate (GDP). The bound form is localized to the nucleus.

Immunogen

denatured recombinant human Ran.

Application

Monoclonal Anti-Ran antibody produced in mouse has been used in:

• western blotting
• immunoprecipitation
• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry

Biochem/physiol Actions

Ran plays an important function in the directionality of nuclear transport. In this form it is important for the termination of the import reaction which is mediated by a complex of the cargo protein with importin-β and its adapter protein importin-α. The binding of RanGTP to importin-β causes the release of the cargo protein from the importin-α/importin-β complex. The import receptor (importin-β or transportin) is then exported out of the nucleus as a RanGTP complex and is released into the cytoplasm when RanGTP is converted to RanGDP by Ran-GAP1 (GTPase-activating protein). Export receptors respond in the opposite way by binding cargo with much higher affinity in the presence of RanGTP and exporting them out of the nucleus as RanGTP complexes. The exported cargo is released when Ran GAP1 converts RanGTP to RanGDP. In order to maintain the import/export cycle, Ran has to return to the nucleus. This is achieved by a small protein called nuclear transport factor 2 (NTF2) also known as p10, that binds RanGDP in the cytoplasm, and delivers it back into the nucleus. In the nucleus RanGDP encounters RanGEF (guanine nucleotide exchange factor) and undergoes nucleotide exchange to become RanGTP.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.