1-O-n-Octyl-β-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.

Application

用于溶解和分离膜蛋白的非离子型可透析洗涤剂。已证明可提高 2D 凝胶中蛋白质的分离度。
N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It is a mild and non-denaturing detergent for the solubilization and reconstitution of membrane proteins. N-Octylglucoside is easily removed by dialysis. N-Octylglucoside has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.

Preparation Note

Working concentration: 46 mM
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.
Store at 15 to 25 °C. (Store dry!)

Analysis Note

Purity: 99% (from C), homogeneous (GC)

Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C₁₄H₂₈O₆

Other Notes

For life science research only. Not for use in diagnostic procedures.

技术参数 Specifications

assay	99% (GC)
mol wt	micellar avg mol wt 25,000, 292.4
packaging	pkg of 10 g
manufacturer/tradename	Roche
aggregation number	84
technique(s)	dialysis: suitable
CMC	20-25 mM (20-25°C)
transition temp	cloud point >100 °C
shipped in	ambient
SMILES string	CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
InChI	1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1
InChI key	HEGSGKPQLMEBJL-RKQHYHRCSA-N

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