- 商品介绍
- 规格参数
- 包装参数
Application
TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.
Biochem/physiol Actions
Binds specifically to mediate neurotrophins BDNF and NT-4/5.
Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.
TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.
Physical form
Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.
Preparation Note
Reconstitute to 100 μg/mL in phosphate buffered saline.
Analysis Note
Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.
Other Notes
Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.
| biological source | human |
| Quality Level | 200 |
| recombinant | expressed in NSO cells |
| assay | >90% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.1-0.4 μg/mL ED50 |
| mol wt | 120-130 kDa by SDS-PAGE (reducing),calculated mol wt 71 kDa |
| packaging | pkg of 100 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| technique(s) | western blot: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | Q16620 |
| storage temp. | −20°C |
| Gene Information | human ... NTRK2(4915) |
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