规格
Accession Number:	NM_001118
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	CHO-K1
Cell State:	Division-Arrested Cells
Druggable Target:	GPCRs
Gene Symbol:	ADCYAP1R1
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	ADCYAP1R1, PAC1
Detection Method:	Fluorescent

储存

K1619 GeneBLAzer® PAC1 CHO-K1 DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® PAC1 CHO-K1 DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• GeneBLAzer® PAC1 CHO-K1 DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® PAC1 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

The GeneBLAzer® PAC1-CRE-bla CHO-K1 cells contain the human Adenylate Cyclase Activating Polypeptide 1 Receptor 1 (ADCYAP1R⁄PAC1) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat #K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE). The PAC1-CRE-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of established ligands. In addition, PAC1-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

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