BIOTIN-14-DCTP 50 NMOL,19518018,Invitrogen

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订货号 9CG5626
品牌型号 Invitrogen 19518018
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最小订货量 1套
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产品介绍 Product Description
规格
Label Type: Biotin & Other Haptens
Labeling Method: Direct Labeling
Label or Dye: Biotin
Shipping Condition: Dry Ice
Concentration: 0.4 mM
储存
Biotin-14-dCTP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.
描述

Biotin-14-dCTP is used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling. Advantages of using biotinylated DNA probes include:

• Convenience and safety—probe preparation generates no radioactive waste
• Stability—probes remain active longer than isotopically labeled probes
• Versatility—biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding

About Biotin-14-dCTP
Biotin-14-dCTP is a patented dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50 µg of DNA.

Performance and quality testing
Purity is evaluated by reverse-phase HPLC.

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