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PrimeFlow™ microRNA Pretreatment Concentrate (4X): cat. 00-16009, 25 mL, store at 2-8°C PrimeFlow™ microRNA Pretreatment Diluent: cat. 00-16008, 75 mL, store at 2-8°C |
This PrimeFlow™ microRNA Pretreatment Buffer is designed to be used in combination with the PrimeFlow™ RNA Assay (cat. no. 88-18005-204) for the optimal detection of microRNA and other small RNA by flow cytometry. Because microRNA molecules have a limited number of target probe binding sites per molecule, we recommend using Type 1, Alexa Fluor™ 647 or Type 10, Alexa Fluor™ 568 Target Probe sets for optimal sensitivity. This buffer may also be used in combination with the PrimeFlow™ Fixation/Permeabilization Buffer Set (cat. no. 88-17000-210) to evaluate antibody performance for intracellular targets. Follow the protocol that is attached to this data sheet. Please note that additional changes in antibody performance may be observed after the hybridization steps of the PrimeFlow RNA Assay.
The PrimeFlow™ RNA Assay and Target Probe Sets for genes of interest are sold separately.