T-REX TANGO P2RY14-BLA U2OS CE KIT,K1858,Invitrogen

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订货号 8AE9019
品牌型号 Invitrogen K1858
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Accession Number: NM_014879.2
Assay Entry: Cell-based beta-arrestin recruitment
Cell Line: U2OS
Cell State: Dividing Cells
Cytoplasmic Tail: Native Tail
Druggable Target: GPCRs
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Population Status: Single Cell Clone Origin
Product Line: Tango™
Readout: End Point
Reporter Gene: BLA (Beta-Lactamase)
System Type: Tango™
Target Entry: P2RY14
Technique: FRET
Detection Method: Fluorescent
储存
Tango™ cell lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
描述

T-REx™ Tango™ P2RY14-bla U2OS cells contain the human purinergic receptor P2Y14 (P2RY14) under doxycycline inducible control linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The T-REx™ Tango™ P2RY14-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of UDP-glucose. In addition, T-REx™ Tango™ P2RY14-bla U2OS cells have been tested for assay performance under variable conditions.

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