规格
|
Final Product Type:
|
Total RNA |
For Use With (Application):
|
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction |
Format:
|
Spin Column |
Isolation Technology:
|
Silica Spin Column |
No. of Reactions:
|
50 Preps |
Quantity:
|
50 |
Sample Type:
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Blood |
Yield:
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1000 µg |
High-throughput Compatibility:
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Not High-throughput Compatible (Manual) |
Purification Target:
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Total RNA |
Purification Time:
|
20 min |
Scale:
|
Mini |
Shipping Condition:
|
Room Temperature |
Starting Material Amount:
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Up to 3.5 x 10^6 leukocytes, 50 to 500 µl blood |
System Type:
|
PureLink™ |
Product Line:
|
PureLink™ |
储存
|
Store at room temperature. |
PureLink® Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 µl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes
Simple RNA Purification from Whole Blood
PureLink® Total RNA Blood Kit is designed for purifying from 50 to 500 µl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.
Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink® Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink® Total RNA Blood Kit.
Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
• Northern Blotting Kits & Reagents
• Nuclease protection assays
• Microarray
• cDNA synthesis
• Real-Time PCR (qPCR)
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.