规格
|
Exonuclease Activity:
|
3' - 5' |
Fidelity (vs. Taq):
|
26 X |
GC-Rich PCR Performance:
|
Low |
Hot Start:
|
Built-In Hot Start |
Overhang:
|
Blunt |
Polymerase:
|
AccuPrime™ Pfx DNA Polymerase |
Product Line:
|
AccuPrime™ |
Reaction Format:
|
SuperMix or Master Mix |
Reaction Speed:
|
Standard |
Shipping Condition:
|
Approved for shipment on Wet or Dry Ice |
Size (Final Product):
|
15 kb or less |
储存
|
• 4 × 1.125 mL AccuPrime Pfx SuperMix
Store at -20°C in a non-frost-free freezer. |
AccuPrime™ Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half. With AccuPrime™ Pfx SuperMix you will:
• Achieve >26-times higher fidelity than with Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization
• Produce products with blunt ends
Using AccuPrime™ Pfx SuperMix
AccuPrime™ Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime™ proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.