cis-Repressed pQE Kan Vector Set，32943，Qiagen，凯杰

The vectors in the cis-Repressed pQE Vector Set are similar to those in the N-Terminus pQE Vector Set, but additionally express the laclq gene product that represses protein expression prior to IPTG induction. This eliminates the need to include this gene on another plasmid. Use of these vectors is recommended when expressing gene products toxic to E. coli. 

• 严谨调控的蛋白表达，增强蛋白结构的细胞内稳定性
• 不需要第二个质粒的反式抑制子
• 氨苄青霉素或卡那霉素抗性
• 卡那霉素抗性确保可靠的克隆，以及更稳定的蛋白表达

Features	Specifications
Tag	6xHis tag
Antibiotic resistance	cis-Repressed pQE Vector Set: Ampicillin; cis-Repressed pQE KAN Vector Set: Kanamycin
Expression species	E. coli
N- or C-terminal tag	N-terminal tag
Expression	In vivo
Special features	Additional expression of the laclq gene
In-frame cloning necessary	Yes
Tag removal sequence	No
All three reading frames provided	Yes

The pQE vectors in this product provide high-level expression of protein in E. coli , enabling:

• Purification of functional proteins under native conditions
• Purification under denaturing conditions for antibody generation
• Efficient detection using Anti·His antibodies and conjugates