规格
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Delivery Type:
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Transfection |
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Key Functions:
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Reporter Assays |
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Product Line:
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Pierce™ |
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Promoter:
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EF-1a |
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Reporter Gene:
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TurboLuc™16 Luciferase |
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Cloning Method:
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Restriction Enzyme ⁄ MCS |
储存
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Store in freezer (-5 to -30°C). |
The Thermo Scientific™ pEF1α Tluc16 Vector encodes the smallest known luciferase, TurboLuc™16 (Tluc16) luciferase, as a reporter under a modified version of the Elongated Factor 1α (EF1α) promoter for constitutive Tluc16 luciferase expression in mammalian cells.
• Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
• Truncated EF1α promoter for constitutive Tluc16 luciferase expression
Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.