规格
|
Label Type:
|
Other Label(s) or Dye(s) |
Label or Dye:
|
AMC (7-amino-4-methylcoumarin) |
Product Line:
|
EnzChek® |
Shipping Condition:
|
Room Temperature |
Detection Method:
|
Fluorescent |
For Use With (Equipment):
|
Fluorometer, Microplate Reader |
Format:
|
Cuvettes, 96-well plate |
储存
|
Store in freezer (-5 to -30°C) and protect from light. |
The EnzChek Caspase-3 Assay Kit #1 allows detection of apoptosis by providing a simple and reliable method for assaying caspase-3 activity. The basis for the assay is the aminomethylcoumarin (AMC)-derived substrate Z-DEVD-AMC. This substrate, which is weakly fluorescent in the UV range (excitation/emission maxima ~330/390 nm), yields a bright, blue-fluorescent product (excitation/emission maxima ~342/441 nm) upon proteolytic cleavage. The kit can be used to continuously measure the activity of caspase-3 and closely related proteases in cell extracts and purified enzyme preparations, using a fluorometer or fluorescence microplate reader.