ENZCHEK CASPASE-3 ASSA 1 KIT,E13183,Invitrogen

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订货号 5TZ2326
品牌型号 Invitrogen E13183
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Label Type: Other Label(s) or Dye(s)
Label or Dye: AMC (7-amino-4-methylcoumarin)
Product Line: EnzChek®
Shipping Condition: Room Temperature
Detection Method: Fluorescent
For Use With (Equipment): Fluorometer, Microplate Reader
Format: Cuvettes, 96-well plate
储存
Store in freezer (-5 to -30°C) and protect from light.
描述

The EnzChek Caspase-3 Assay Kit #1 allows detection of apoptosis by providing a simple and reliable method for assaying caspase-3 activity. The basis for the assay is the aminomethylcoumarin (AMC)-derived substrate Z-DEVD-AMC. This substrate, which is weakly fluorescent in the UV range (excitation/emission maxima ~330/390 nm), yields a bright, blue-fluorescent product (excitation/emission maxima ~342/441 nm) upon proteolytic cleavage. The kit can be used to continuously measure the activity of caspase-3 and closely related proteases in cell extracts and purified enzyme preparations, using a fluorometer or fluorescence microplate reader.

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