AllPrep DNA/RNA Kits enable simultaneous purification of genomic DNA and total RNA from each cell or tissue sample. Since there is no need to divide each sample into two for separate purification procedures, maximum yields of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately. The AllPrep DNA/RNA Micro and Mini Kits can be automated on the QIAcube Connect. The AllPrep DNA/RNA 96 Kit is well suited for high-throughput purification of genomic DNA and total RNA from up to 96 samples. In partnership with My Green Lab, we've also assessed the environmental impact of the AllPrep DNA/RNA Mini Kit (50) and the QIAwave DNA/RNA Mini Kit (50). My Green Lab ACT environmental impact factor labels aim to assess and rate products based on various sustainability criteria, including: Manufacturing Responsible chemical management Sustainable content within products and packaging materials Disposal of the packaging at the end of life Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact (see figures " AllPrep DNA/RNA Mini Kit (50) environmental impact factor label US , EU and UK ") and “QIAwave DNA/RNA Mini Kit (50) ACT environment impact factor label US , EU and UK ”).
- High-quality DNA and RNA from the same sample
- Maximal yields of DNA and RNA from precious samples
- Rapid purification with short, streamlined protocol
- Streamlined protocols for 96-well plates
- Ready-to-use DNA and RNA for any downstream analysis
The purified genomic DNA has an average length of 15–30 kb, depending on homogenization conditions, and is suited for any application, including next-generation sequencing, Southern-, dot-, and slot-blot analyses; and PCR and multiplex PCR.
The purified total RNA can be used in any application, such as RNA-seq RT-PCR and real-time RT-PCR; differential display; cDNA synthesis; northern-, dot-, and slot-blot analyses; and microarrays.
A supplementary protocol allows optional purification of protein. The purified protein is denatured and can be used in applications such as SDS-PAGE, western blotting, and 2D gel electrophoresis.