规格
|
In Gel Dye:
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Proprietary Stain |
Mode of Separation:
|
Molecular Weight |
Product Line:
|
E-Gel™ |
Sample Buffer Type:
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Preparative without dyes, Non-preparative |
Sample Loading Volume:
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20 µL |
Sample Type:
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Double-Stranded DNA (dsDNA), Single-Stranded RNA |
Shipping Condition:
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Room Temperature |
Well Design:
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Single Comb |
Kit Contents:
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E-Gel EX 20-Paks contain 20 gels. |
Separation Range:
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100 bp to 2 kb |
Gel Percentage:
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2 % |
Gel Type:
|
E-Gel |
Wells:
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11 wells |
Quantity:
|
20 Pack |
储存
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E-Gel® EX 20-Paks contain 20 gels. Store gels at room temperature. E-Gel® EX gels are guaranteed stable for 3 months when properly stored. |
Invitrogen E-Gel EX precast agarose gels are designed for ultrasensitive and convenient DNA and RNA sample electrophoresis as fast as in 10–15 minutes. Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run. E-Gel agarose gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, or for DNA fragment library analysis. E-Gel agarose gels are also suitable for a quick check of RNA samples.
Features of E-Gel EX agarose gels include:
• Instantaneous setup—precast E-Gel agarose gels are ready for use when you are, and do not require gel preparation or additional buffers
• High sensitivity—detect 1 ng of sample DNA
• Rapid analysis—complete DNA sample separation in less than 10–15 minutes
• In-process control—view DNA sample migration in real time
• Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
• For DNA and RNA—ideal for DNA sample analysis, and suitable for quick check of RNA samples
Ultra-sensitive detection of DNA
E-Gel EX gels pre-stained with SYBR Gold DNA stain were developed for ultimate sensitivity and demonstrate up to 5-fold greater sensitivity than comparable gels containing ethidium bromide. This superior sensitivity allows you to use lower amounts of sample, saving time and money.
Fast and convenient analysis
E-Gel EX gels are our fastest-resolving agarose gels, enabling sample analysis in as little as 10–15 minutes. Each E-Gel agarose gel contains agarose, electrodes, SYBR Gold DNA stain, and a bufferless ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not required any gel or buffer preparation or gel straining steps. Just load your samples and run.
Live monitoring of DNA migration
The E-Gel EX agarose gels are pre-stained with SYBR Gold II DNA gel stain with an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, safe real-time monitoring of DNA or RNA migration is enabled without risk to eyes or possible damage of the sample, unlike UV illumination.
Quick check of RNA samples
Besides DNA analysis, E-Gel EX gels can also be used for fast analysis of RNA samples to check their integrity before proceeding with downstream applications.
Easy access to the gel
E-Gel cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), for easy excision of specific bands or transfer of the gel to a membrane for southern blot analysis.
Nucleic acid markers
For optimal DNA analysis on E-Gel agarose gels, we recommend specially formulated E-Gel 1 Kb Plus Express DNA Ladder (Cat. No. 10488091) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099) or E-Gel Ultra Low DNA Ladder (Cat. No. 10488096). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.
Learn more about our E-Gel DNA ladders ›
For RNA sample analysis on E-Gel agarose gels, we recommend Millennium RNA Marker (Cat. No. AM7150) or Century-Plus RNA Ladder (Cat. No. AM7145).
Running and imaging devices
E-Gel EX gels require the Invitrogen E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.
Learn more about the E-Gel Power Snap Electrophoresis System ›
Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 11-well E-Gel EX agarose gels are available in 1%, 2%, and 4% gel percentages. E-Gel agarose gel selection guide