LifeGuard Soil Preservation (1000 ml)，12868-1000，Qiagen，凯杰

The QIAseq Targeted RNAseq Panel for T-cell Receptor enables targeted next-generation sequencing (NGS) of the human or mouse expressed T-cell receptor (TCR), which includes four distinct genes: TCR-alpha, TCR-beta, TCR-gamma and TCR-delta. This highly optimized solution incorporates unique molecular indices (UMIs) to facilitate ultrasensitive and accurate characterization of the immune repertoire in cells and tissues starting from 200 pg to 1000 ng of total RNA. The adaptive immune system is composed of T and B lymphocytes that bind antigens via highly specific T-cell receptors (TCRs) and B cell receptors (BCRs) on their cell surfaces. To recognize a nearly infinite number of potential antigens, extensive sequence diversity of TCRs and BCRs is generated by somatic V(D)J recombination of the TCR and BCR loci, and by subsequent somatic hypermutation and class switch recombination of the BCR upon antigen stimulation. Accurate characterization of the TCR and BCR repertoires is key to understanding adaptive immune responses and has many applications across different fields, including vaccine development, autoimmunity, monitoring treatment response in lymphoid malignancies and immunotherapy. Compared to traditional methods, NGS provides an unprecedented, high-resolution picture of the immune repertoire. However, many available immune repertoire sequencing methods involve multiplex PCR with primers targeting different V or J regions, which can introduce substantial amplification bias. The QIAseq Targeted RNAseq Panel for T-cell Receptor utilizes unique molecular indices (UMIs) with QIAseq Enrichment Technology to robustly create targeted RNA-seq libraries for NGS instruments. This library construction approach greatly improves amplification uniformity compared to multiplexed PCR-based V and J primer pools. The incorporation of UMIs before the amplification step further reduces amplification bias and allows for accurate and sensitive TCR clonotype and repertoire diversity assessment. The QIAseq Targeted RNAseq Panel for T-cell Receptor is a complete Sample to Insight solution for precise characterization of the TCR immune repertoire using NGS. The purchase of the TCR Panel includes access to GeneGlobe Analyze, which contains a highly optimized pipeline for read mapping and clonotype calling.     

• Unique molecular indexing (UMI) technology removes amplification, duplication and sequencing artifacts to increase accuracy
• Profile single TCR receptors (TCR-alpha, TCR-beta, TCR-gamma, TCR-delta) or combine panels for a comprehensive immune repertoire
• Single-tube library construction and versatile panels allow analysis of one, two, three or all four receptors at once
• Unique dual indexing (UDI) minimizes sample index misassignment
• Includes access to GeneGlobe Analyze for data analysis, including read alignment and clonotype report